| Year: | 2000 |
| Team: | Appalachian State Mountaineers |
| Sport: | football |
| Conference: | Southern Conference |
| Short Conf: | SoCon |
| Tsnrank: | 4 |
| Record: | 10–4 |
| Conf Record: | 6–2 |
| Head Coach: | Jerry Moore |
| Hc Year: | 12th |
| Stadium: | Kidd Brewer Stadium |
| Bowl: | NCAA Division I-AA Semifinal |
| Bowl Result: | L 16–19 vs. Montana |
The 2000 Appalachian State Mountaineers football team was an American football team that represented Appalachian State University as a member of the Southern Conference (SoCon) during the 2000 NCAA Division I-AA football season. In their 12th year under head coach Jerry Moore, the Mountaineers compiled an overall record of 10–4, with a conference mark of 6–2, and finished as SoCon co-champion. Appalachian State advanced to the NCAA Division I-AA Football Championship playoffs, where they upset Troy State in the first round, Western Kentucky in the quarterfinals, and lost to Montana in the semifinals.