| Season: | 2000–01 |
| Nextseason: | 2001–02 |
The 2000–01 Ukrainian First League was the tenth season of the Ukrainian First League which was won by FC Dynamo-2 Kyiv. The season started on July 23, 2000, and finished on June 28, 2001.
Three clubs promoted from the 1999-2000 Ukrainian Second League.
Three clubs were relegated from the 1999-00 Ukrainian Top League:
In 2000-01 season, the Ukrainian First League consists of the following teams:
Statistics are taken from here.[1]
| Scorer | Goals (Pen.) | Team | |
|---|---|---|---|
| 1 |  Serhiy Chuychenko | 20 (8) | Polihraftekhnika Oleksandriya |
| 2 | Serhiy Bohatyryov | 13 (7) | Zakarpattia Uzhhorod |
| 3 | Oleksiy Ivanov | 11 | Polihraftekhnika Oleksandriya |
| 4 | Vadym Oliynyk | 10 (7) | Metalurh Nikopol |
| 5 | Myroslav Bundash | 8 | Zakarpattia Uzhhorod |
| Valentyn Hrehul | 8 (5) | Prykarpattia Ivano-Frankivsk | |
| 7 | Mykola Kovalchuk | 7 | CSKA-2 / Vinnytsia |
| Serhiy Shubin | 7 | FC Vinnytsia | |
| Serhiy Dranytskyi | 7 (3) | Chornomorets Odesa | |
| Oleh Mazurenko | 7 (4) | SC Mykolaiv |