| Nextseason: | 2001–02 |
The 2000–01 Scottish Second Division was won by Partick Thistle who, along with second placed Arbroath, were promoted to the First Division. Queen's Park and Stirling Albion were relegated to the Third Division.
The average attendance for Scottish Second Division clubs for season 2000/01 are shown below:
| Club | Average | |
|---|---|---|
| Partick Thistle | 2,917 | |
| Queen of the South | 1,271 | |
| Queen's Park | 1,141 | |
| Arbroath | 805 | |
| Stirling Albion | 787 | |
| Forfar Athletic | 625 | |
| Berwick Rangers | 609 | |
| Stenhousemuir | 595 | |
| Stranraer | 535 | |
| Clydebank | 361 |