| Competition: | NOFV-Oberliga |
| Season: | 2000–01 |
| Winners: | Berliner FC Dynamo, 1. FC Magdeburg |
| Promoted: | 1. FC Magdeburg |
| Relegated: | Tennis Borussia Berlin (A), FC Anker Wismar, SV Schwarz-Rot Neustadt, SD Croatia Berlin, VfL Halle 1896, FC Anhalt Dessau, Bischofswerdaer FV 08, FSV Wacker 90 Nordhausen |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The 2000–01 season of the NOFV-Oberliga was the seventh season of the league at tier four (IV) of the German football league system.
The NOFV-Oberliga was split into two divisions, NOFV-Oberliga Nord and NOFV-Oberliga Süd. The champions of each, BFC Dynamo and 1. FC Magdeburg, entered into a play-off against each other for the right to play in the 2001–02 Regionalliga Nord. 1. FC Magdeburg won 5–2 over two legs and thus gained promotion.
| BFC DYNAMO: | ||||
| GK | Nico Thomaschewski | |||
| DF | Florin Batrinu | |||
| DF | ||||
| DF | ||||
| DF | Jörn Lenz | |||
| DF | Dorel Zegrean | |||
| MF | ||||
| MF | Sebastian Hahn | |||
| MF | ||||
| FW | ||||
| FW | Danut Oprea | |||
| Substitutes: | ||||
| MF | ||||
| FW | ||||
| Manager: | ||||
| Jürgen Bogs | ||||
| 1. FC MAGDEBURG: | ||||
| GK |  Miroslaw Dreszer | |||
| DF | Maik Franz | |||
| DF | Sören Holz | |||
| DF | ||||
| DF | Bodo Schmidt | |||
| MF | ||||
| MF | Andreas Lücke | |||
| MF |  Petr Maslej | |||
| MF | ||||
| FW | ||||
| FW | ||||
| Substitutes: | ||||
| DF | ||||
| MF | ||||
| FW | ||||
| Manager: | ||||
| Eberhard Vogel | ||||
| 1. FC MAGDEBURG: | ||||
| GK | Miroslaw Dreszer | |||
| DF | Maik Franz | |||
| DF | Sören Holz | |||
| DF | Waldemar Koc | |||
| DF | Bodo Schmidt | |||
| MF | ||||
| MF | ||||
| MF | ||||
| MF |  Armando Zani | |||
| FW | Josef Ivanovic | |||
| FW | ||||
| Substitutes: | ||||
| MF | ||||
| FW | ||||
| FW | ||||
| Manager: | ||||
| Eberhard Vogel | ||||
| BFC DYNAMO: | ||||
| GK | Nico Thomaschewski | |||
| DF | ||||
| DF | ||||
| DF | Mario Kallnik | |||
| DF | Jörn Lenz | |||
| DF | Dorel Zegrean | |||
| MF | Silvian Cristescu | |||
| MF | ||||
| MF | ||||
| FW |  Denis Koslov | |||
| FW | ||||
| Substitutes: | ||||
| MF | ||||
| FW | ||||
| FW | ||||
| Manager: | ||||
| Jürgen Bogs | ||||