| Season: | 2000–01 |
| Winners: | Rushden & Diamonds (1st Football Conference title) |
| Continentalcup1: | Direct promotion |
| Continentalcup2: | Conference League Cup winners |
| Continentalcup3: | FA Trophy winners |
| Continentalcup3 Qualifiers: | Canvey Island (Isthmian League) (1st FA Trophy title) |
| League Topscorer: | Duane Darby (Rushden & Diamonds), 24 |
| Biggest Home Win: | Hednesford – Northwich Victoria 7–1 (2 September 2000) |
| Biggest Away Win: | Kingstonian – Morecambe 1–6 (10 March 2000) |
| Highest Scoring: | Leigh RMI – Nuneaton 6–2 (3 March 2001); Boston – Hereford 5–3 (5 May 2001); Stevenage – Telford United 5–3 (30 September 2000); Forest Green Rovers – Dagenham & Redbridge 4–4 (16 September 2000); Morecambe – Scarborough 4–4 (16 December 2000); Woking– Dagenham & Redbridge 4–4 (14 April 2001) |
| Matches: | 462 |
| Total Goals: | 1256 |
| Longest Wins: | ? |
| Longest Unbeaten: | ? |
| Longest Losses: | ? |
| Highest Attendance: | Yeovil Town v Rushden & Diamonds, 8,868 (21 April 2001) |
| Lowest Attendance: | ? |
| Average Attendance: | 1,553 (– 4% compared to the previous season) |
| Nextseason: | 2001–02 |
The Football Conference season of 2000–01 was the twenty-second season of the Football Conference, also known as the Nationwide Conference for sponsorship reasons.
| width=20 | Rank | width=155 | Player | width=155 | Club | width=50 | League | width=50 | FA Cup | width=50 | FA Trophy | width=50 | League Cup | width=50 | Total |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Duane Darby | Rushden & Diamonds | 24 | 0 | 3 | 0 | 27 | ||||||||
| 2 | 19 | 1 | 0 | 1 | 21 | ||||||||||
| 3 | 18 | 3 | 0 | 1 | 22 | ||||||||||
| = | Rushden & Diamonds | 18 | 1 | 1 | 0 | 20 | |||||||||
| 5 | 15 | 0 | 1 | 0 | 16 | ||||||||||
| 6 | 14 | 0 | 4 | 0 | 18 | ||||||||||
| 7 | 13 | 0 | 5 | 0 | 18 | ||||||||||
| = | 13 | 3 | 1 | 0 | 17 | ||||||||||
| = | 13 | 1 | 0 | 2 | 16 | ||||||||||
| = | 13 | 1 | 1 | 0 | 15 | ||||||||||
| = | 13 | 0 | 0 | 1 | 14 | ||||||||||
| = | 13 | 0 | 0 | 0 | 13 | ||||||||||
| = | 13 | 0 | 0 | 0 | 13 | ||||||||||
| 14 | 12 | 1 | 1 | 3 | 17 | ||||||||||
| = | 12 | 3 | 1 | 1 | 17 | ||||||||||
| = | 12 | 2 | 0 | 0 | 14 | ||||||||||
| = | 12 | 1 | 0 | 0 | 13 | ||||||||||
| = | 12 | 0 | 0 | 0 | 12 | ||||||||||
| 19 | 11 | 0 | 4 | 2 | 17 | ||||||||||
| = | 11 | 0 | 0 | 0 | 11 | ||||||||||
| = | 11 | 0 | 0 | 0 | 11 | ||||||||||
| = | 11 | 0 | 0 | 0 | 11 |