| Competition: | Druga HNL |
| Season: | 2000–01 |
| Winners: | Kamen Ingrad |
| Promoted: | Kamen Ingrad Pomorac Kostrena Zadar TŠK Topolovac |
| Relegated: | Jadran Poreč |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The 2000–01 Druga HNL was the 10th season of Druga HNL, the second level league in Croatian football. The format of the league was unchanged from the 1999–2000 season. A total of 18 clubs competed in Druga HNL this season, in a double round-robin format.
Due to the expansion of top level Prva HNL set for the 2001–02 season, four clubs were promoted at the end of season (Kamen Ingrad, Pomorac Kostrena, Zadar and TŠK Topolovac, while Solin entered promotion playoff). Also, Druga HNL was split into two divisions the following season.
| Club | City / Town | Stadium | Capacity | 1999–2000 result | |
|---|---|---|---|---|---|
| Belišće | Belišće | Gradski stadion Belišće | 4,000 | ||
| Bjelovar | Bjelovar | Gradski stadion Bjelovar | 4,000 | ||
| Croatia Sesvete | Sesvete | Stadion ŠRC Sesvete | 3,500 | ||
| Imotska Krajina | Proložac | Stadion Šarampov | 1,000 | ||
| Istra Pula | Pula | Stadion Aldo Drosina | 7,000 | ||
| Jadran Poreč | Poreč | Stadion Veli Jože | 2,000 | ||
| Kamen Ingrad | Velika | Stadion Kamen Ingrada | 8,000 | ||
| Koprivnica | Koprivnica | Gradski stadion u Koprivnici | 3,400 | ||
| Mosor | Žrnovnica | Igralište Pricviće | 3,000 | ||
| Orijent | Rijeka | Stadion SC Krimeja | 4,000 | ||
| Pomorac | Kostrena | Stadion Žuknica | 3,500 | ||
| Segesta | Sisak | Gradski stadion Sisak | 8,000 | ||
| Solin Građa | Solin | Stadion pokraj Jadra | 4,000 | ||
| TŠK Topolovac | Topolovac | Park grofova | 1,000 | ||
| PIK Vrbovec | Vrbovec | Gradski stadion kraj Sajmišta | 3,000 | ||
| Vukovar '91 | Vukovar | Gradski stadion Borovo Naselje | 6,000 | ||
| Zadar | Zadar | Stadion Stanovi | 5,860 | ||
| Žminj | Žminj | Igralište Žminja | 1,000 |