| Competition: | Welsh Alliance League |
| Season: | 2000–01 |
| Winners: | Llanfairpwll |
| Matches: | 182 |
| Total Goals: | 821 |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The 2000–01 Welsh Alliance League is the 17th season of the Welsh Alliance League, which is in the third level of the Welsh football pyramid.
The league consists of fourteen teams and concluded with Llanfairpwll as champions and promoted to the Cymru Alliance.
Halkyn United were champions in the previous season and were promoted to the Cymru Alliance.
Llandyrnog United were relegated to the Clwyd League and replaced by Gwynedd League champions, Bethesda Athletic and Clwyd League champions, Abergele Town.
| Team | Location | Ground | |
|---|---|---|---|
| Abergele Town | Maesdu Park | ||
| Lôn Bach | |||
| Bangor City Reserves | Farrar Road Stadium | ||
| Parc Meurig | |||
| Lon yr Ysgol | |||
| Colwyn Bay YMCA | |||
| Y Morfa Stadium | |||
| Cae Ffwt | |||
| Arriva Ground | |||
| Maes Eilian | |||
| Ffordd Padarn | |||
| Penmaenmawr Phoenix | Cae Sling | ||
| Bastion Road | |||
| Rhyl Reserves | Belle Vue |