| Competition: | Tunisian Ligue Professionnelle 1 |
| Season: | 2000–01 |
| Winners: | Espérance de Tunis |
| Relegated: | AS Djerba JS Kairouan |
| Continentalcup1: | Champions League |
| Continentalcup1 Qualifiers: | Espérance de Tunis |
| Continentalcup2: | Cup Winners' Cup |
| Continentalcup2 Qualifiers: | CS Hammam-Lif |
| Continentalcup3: | CAF Cup |
| Continentalcup3 Qualifiers: | Étoile du Sahel |
| League Topscorer: | Sallemi (11 goals) |
| Biggest Home Win: | CSS 5-0 ASD |
| Biggest Away Win: | JSK 1-4 CA |
| Highest Scoring: | USM 4-3 ASD JSK 3-4 CSHL CSHL 4-3 ESZ |
| Matches: | 132 |
| Total Goals: | 296 |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The 2000–01 Tunisian Ligue Professionnelle 1 season was the 75th season of top-tier football in Tunisia.