| Competition: | VI Torneo Argentino A |
| Season: | 2000–01 |
| Winners: | Huracán (TA) (1st divisional title) |
| Promoted: | Huracán (TA) |
| Relegated: | Deportivo Barraca (PdlL) General Belgrano Liniers (BB) |
| Prevseason: | 1999–00 |
| Nextseason: | 2001–02 |
The 2000–01 Argentine Torneo Argentino A[1] was the sixth season of third division professional football in Argentina. A total of 18 teams competed; the champion was promoted to Primera B Nacional.
| Club | City | Stadium | |
|---|---|---|---|
| Aldosivi | Mar del Plata | José María Minella | |
| Comodoro Rivadavia | Estadio Municipal | ||
| Cultural Argentino | General Pico | El Volcán | |
| General Belgrano | Santa Rosa | Nuevo Rancho Grande | |
| Huracán | San Rafael | Pretel Hermanos | |
| Huracán | Tres Arroyos | Roberto Lorenzo Bottino | |
| Juventud Alianza | Santa Lucía | Bosque del Bajo Grande | |
| Liniers | Bahía Blanca | Alejandro Pérez | |
| Luján de Cuyo | Luján de Cuyo | Jardín del Bajo |
| Club | City | Stadium | |
|---|---|---|---|
| 13 de Junio | Pirané | Pirané | |
| Ben Hur | Rafaela | Parque Barrio Ilolay | |
| Deportivo Barraca | Paso de los Libres | Agustín Faraldo | |
| Douglas Haig | Pergamino | Miguel Morales | |
| Gimnasia y Tiro | Salta | Gigante del Norte | |
| Huracán Corrientes | Corrientes | José Antonio Romero Feris | |
| Ñuñorco | Monteros | Ñuñorco | |
| Patronato | Paraná | Presbítero Bartolomé Grella | |
| Tiro Federal | Rosario | Fortín de Ludueña |
|-!colspan="5"|Relegation/promotion playoff 1|-!colspan="5"|Relegation/promotion playoff 2