| Sex: | none |
| Mode: | Women's Basketball |
| Year: | 2000–01 |
| Prev Year: | 1999–2000 |
| Next Year: | 2001–02 |
| Team: | Southwest Missouri State Lady Bears |
| Conference: | Missouri Valley Conference |
| Short Conf: | MVC |
| Coachrank: | 4 |
| Aprank: | 15 |
| Record: | 29–6 |
| Conf Record: | 16–2 |
| Head Coach: | Cheryl Burnett |
| Hc Year: | 14th |
| Asst Coach1: | Marla Douglass |
| Asst Coach2: | Jim Middleton |
| Asst Coach3: | Lynette Robinson |
| Arena: | Hammons Student Center |
| Champion: | MVC tournament champions MVC regular season co-champions |
| Tourney: | NCAA tournament |
| Tourney Result: | Final Four |
The 2000–01 Southwest Missouri State Lady Bears basketball team represented Southwest Missouri State University during the 2000–01 NCAA Division I women's basketball season. The Lady Bears, led by 14th-year head coach Cheryl Burnett, played their home games at Hammons Student Center and were members of the Missouri Valley Conference.
They finished the season 29–6 and 16–2 in conference play to tie for the regular season MVC title. As the number two seed in the conference tournament, they defeated Indiana State, Northern Iowa, and Drake to take home the MVC tournament championship. They received an automatic bid to the NCAA tournament and were assigned the No. 5 seed in the West region. They defeated No. 12 seed Toledo, No. 4 seed Rutgers, No. 1 seed Duke, and No. 6 seed Washington to reach the second Final Four in program history. The Lady Bears were defeated by Purdue in the National semifinals.[1]
Southwest Missouri State spent the entire season ranked in both major polls. The team was ranked in the top 15 for nine weeks, and had a final ranking in the Coaches poll of No. 4.
|-!colspan=6 style=| Regular season
|-!colspan=6 style=| MVC tournament|-!colspan=6 style=| NCAA tournament
See main article: 2000–01 NCAA Division I women's basketball rankings.