| Competition: | Segunda División |
| Season: | 2000–01 |
| Winners: | Sevilla FC |
| Promoted: | Sevilla Real Betis CD Tenerife |
| Relegated: | SD Compostela Universidad de Las Palmas CF Getafe CF UE Lleida |
| League Topscorer: |  Salva Ballesta |
| Matches: | 462 |
| Total Goals: | 1016 |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The 2000–01 Segunda División season saw 22 teams participate in the second flight Spanish league. The teams that promoted to La Liga (top division) were Sevilla FC, Real Betis and CD Tenerife. The teams that relegated to Segunda División B were SD Compostela, Universidad de Las Palmas CF, Getafe CF and UE Lleida.
| Autonomous community | Number of teams | Teams | |
|---|---|---|---|
| 1 | 5 | Betis, Córdoba, Real Jaén, Recreativo, Sevilla | |
| 2 | 3 | Atlético Madrid, Getafe, Leganés | |
| 3 | 2 | Tenerife, Universidad de Las Palmas | |
| 2 | Badajoz, Extremadura | ||
| 2 | Compostela, Racing de Ferrol | ||
| 2 | Elche, Levante | ||
| 7 | 1 | Sporting | |
| 1 | Eibar | ||
| 1 | Albacete | ||
| 1 | Salamanca | ||
| 1 | Lleida | ||
| 1 | Murcia | ||