| Club: | Rayo Vallecano |
| Season: | 2000–01 |
| Manager: | Juande Ramos |
| League: | La Liga |
| League Result: | 14th |
| Cup1: | Copa del Rey |
| Cup1 Result: | Round of 16 |
| Cup2: | UEFA Cup |
| Cup2 Result: | Quarter-finals |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The 2000–01 season was the 77th season in the history of Rayo Vallecano and the club's second consecutive season in the top flight of Spanish football. In addition to the domestic league, Rayo Vallecano participated in this season's edition of the Copa del Rey and UEFA Cup.
Rayo Vallecano reached the quarter-finals of the UEFA Cup.
Squad at end of season[1]
See main article: 2000–01 La Liga.