| Competition: | Primera B Nacional |
| Season: | 2000–01 |
| Winners: | Banfield (2nd divisional title) |
| Promoted: | Banfield Nueva Chicago |
| League Topscorer: | Daniel Jiménez 23 goals |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The 2000–01 Argentine Primera B Nacional was the 15th season of second division professional of football in Argentina. A total of 30 teams competed; the champion and runner-up were promoted to Argentine Primera División.
The Promotion Playoff was played by the teams placed 1st and 2nd of each zone. The winning team was declared champion and was automatically promoted to Primera División. The teams that lost in semifinal joined into the Quarterfinals of the Second Promotion Playoff, and the team that lost in the final joined in the semifinal of the Second Promotion Playoff.
|-!colspan="5"|Semifinals|-!colspan="5"|Semifinals1: Qualified because of sport advantage.
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Note: Banfield won 6–3 on aggregate, promoting to Primera DivisiónThe Second Promotion Playoff or Torneo Reducido was played by the teams placed 3rd to 6th of each zone. Instituto and Gimnasia y Esgrima (CdU) joined in the Quarterfinals, and Quilmes joined in the Semifinals. The winner was promoted to Primera División.
1: Qualified because of sport advantage.
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The best two teams of each zone that were not promoted (Quilmes and Instituto) played against the 18th and the 17th placed of the Relegation Table of 2000–01 Primera División.
|-!colspan="5"|Relegation/promotion playoff 1|-!colspan="5"|Relegation/promotion playoff 2|-|}
7 teams were relegated: 2 teams with the worst co-efficient from Interior Zone, 2 teams with the worst co-efficient from Metropolitana Zone and 3 more teams regardless their affiliation.
| width=28 | width=185 | Team | width=50 | width=50 | width=50 | width=50 | width=50 | width=50 | Situation | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Instituto | 57 | — | 71 | 128 | 62 | Indirect | ||||||||||
| 2 | Quilmes | 47 | 63 | 50 | 160 | 90 | Direct | ||||||||||
| 3 | Arsenal | 57 | 55 | 44 | 156 | 90 | Direct | ||||||||||
| 4 | Banfield | 43 | 59 | 49 | 151 | 90 | Direct | ||||||||||
| 5 | Gimnasia y Esgrima (J) | — | — | 50 | 50 | 32 | Indirect | ||||||||||
| 6 | Atlético de Rafaela | 44 | 51 | 45 | 140 | 92 | Indirect | ||||||||||
| 7 | Gimnasia y Esgrima (CdU) | 37 | 47 | 55 | 139 | 92 | Indirect | ||||||||||
| 8 | San Martín (M) | 38 | 50 | 51 | 139 | 92 | Indirect | ||||||||||
| 9 | Racing (C) | — | 38 | 52 | 90 | 62 | Indirect | ||||||||||
| 10 | Juventud Antoniana | 46 | 46 | 43 | 135 | 92 | Indirect | ||||||||||
| 11 | San Martín (SJ) | 36 | 49 | 50 | 135 | 92 | Indirect | ||||||||||
| 12 | Almirante Brown (A) | 42 | 40 | 50 | 132 | 92 | Indirect | ||||||||||
| 13 | Atlético Tucumán | 57 | 29 | 43 | 129 | 92 | Indirect | ||||||||||
| 14 | Defensa y Justicia | 44 | 52 | 29 | 125 | 90 | Direct | ||||||||||
| 15 | Villa Mitre | — | 41 | 43 | 84 | 62 | Indirect | ||||||||||
| 16 | Platense | — | 45 | 33 | 78 | 58 | Direct | ||||||||||
| 17 | Independiente Rivadavia | — | 45 | 35 | 80 | 62 | Indirect | ||||||||||
| 18 | Godoy Cruz | 30 | 43 | 44 | 117 | 92 | Indirect | ||||||||||
| 19 | Tigre | 43 | 45 | 26 | 114 | 90 | Direct | ||||||||||
| 20 | El Porvenir | 45 | 50 | 19 | 114 | 90 | Direct | ||||||||||
| 21 | Nueva Chicago | 44 | 33 | 37 | 114 | 90 | Direct | ||||||||||
| 22 | Olimpo | 43 | 41 | 32 | 116 | 92 | Indirect | ||||||||||
| 23 | Central Córdoba (R) | 43 | 38 | 31 | 112 | 90 | Direct | ||||||||||
| 24 | All Boys | 51 | 29 | 30 | 110 | 90 | Primera B Metropolitana | Direct | |||||||||
| 25 | San Miguel | 43 | 35 | 28 | 106 | 90 | Direct | ||||||||||
| 26 | San Martín (T) | 46 | 28 | 32 | 106 | 92 | Torneo Argentino A | Indirect | |||||||||
| 27 | Cipolletti | 53 | 23 | 25 | 101 | 92 | Indirect | ||||||||||
| 28 | — | — | 24 | 24 | 24 | Primera B Metropolitana | Direct | ||||||||||
| 29 | General Paz Juniors | — | — | 29 | 29 | 32 | Torneo Argentino A | Indirect | |||||||||
| 30 | Ferro Carril Oeste | — | — | 18 | 18 | 24 | Primera B Metropolitana | Direct |
Note: Clubs with indirect affiliation with AFA are relegated to the Torneo Argentino A, while clubs directly affiliated face relegation to Primera B Metropolitana. Clubs with direct affiliation are all from Greater Buenos Aires, with the exception of Newell's, Rosario Central, Central Córdoba and Argentino de Rosario, all from Rosario, and Unión and Colón from Santa Fe.