The 2000–01 North West Counties Football League season was the 19th in the history of the North West Counties Football League, a football competition in England. Teams were divided into two divisions: Division One and Division Two.
| Competition: | North West Counties Football League Division One |
| Season: | 2000–01 |
| Winners: | Rossendale United |
| Promoted: | Rossendale United |
| Relegated: | Cheadle Town Leek CSOB |
| Matches: | 462 |
| Total Goals: | 1554 |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
Division One featured three new teams:
| Competition: | North West Counties Football League Division Two |
| Season: | 2000–01 |
| Winners: | Warrington Town |
| Promoted: | Atherton Laburnum Rovers Warrington Town |
| Relegated: | None |
| Matches: | 380 |
| Total Goals: | 1354 |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
Division Two featured four new teams: