| Competition: | Nationalliga A |
| Season: | 2000–01 |
| Winners: | Grasshopper Club 26th title |
| Relegated: | Yverdon Sport |
| League Topscorer: | Stéphane Chapuisat (21) Christian Giménez (21) |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The 2000–01 season Swiss Football League contains two divisions, the Swiss Super League, at the time called Nationalliga A, and the Swiss Challenge League, at the time called Nationalliga B (in French: Ligue Nationale A/B and Italian: Lega Nazionale A/B). At the end of the season, Nationalliga A held its own championship playoffs, and Nationalliga B held its own relegation playoffs. Additionally, the last 4 teams from A and top 4 teams from B held a relegation/promotion playoff.
The Qualification Round to the League season 2000–01 was contested by twelve teams. The first eight teams of the regular season (or Qualification) then competed in the Championship Playoff Round. The teams in the ninth to twelfth positions completed with the top four teams of the Nationalliga B in a Nationalliga A/B Playoff round. The regular season (Grunddurchgang) started on 15 July and ended on 10 December. At the end of the season Grasshopper Club Zürich won the championship.
The first eight teams of the regular season (or Qualification) competed in the Championship Playoff Round. They took half of the points (rounded up to complete units) gained in the Qualification as Bonus with them.
Each team was awarded half of the points from the regular season.
The teams in the ninth to twelfth positions in Nationalliga A competed with the top four teams of Nationalliga B in a Nationalliga A/B Playoff round.