| Competition: | Midland Football Alliance |
| Season: | 2000–01 |
| Winners: | Stourport Swifts |
| Promoted: | Stourport Swifts |
| Relegated: | West Midlands Police |
| Matches: | 462 |
| Total Goals: | 1568 |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The 2000–01 Midland Football Alliance season was the seventh in the history of Midland Football Alliance, a football competition in England.
The league featured 20 clubs from the previous season, along with two new clubs: