| Season: | 2000–01 |
| Total Goals: | 337 |
| Average Goals: | 2.55 |
| Nextseason: | 2001–02 |
The 2000–01 Maltese Second Division (known as Rothmans Second Division 2000-01 due to sponsorship reasons) started on 16 September 2000 and ended on 14 May 2000. In this season the number of participants was dropped from 13 to 12. Gzira United and Zebbug Rangers were relegated from the First Division, while Balzan Youths and Luqa St. Andrews were promoted from the Third. The league was won by Mqabba and Balzan Youths finished as runners-up. Both were promoted to the 2001–02 Maltese First Division. This were two straight promotions for Balzan Youths. Santa Lucia and Siggiewi were relegated to the Maltese Third Division.