| Season: | 2000–01 |
| League Topscorer: | Kevin Loughborough (12) |
| Total Goals: | 270 |
| Average Goals: | 3 |
| Nextseason: | 2001–02 |
The 2000–01 Maltese First Division (known as the Rothmans First Division for sponsorship reasons) started on 9 September 2000 and finished on 29 April 2001. Gozo and Żurrieq were the relegated teams from the 1999–2000 Maltese Premier League. Marsaxlokk and Qormi were the promoted teams from the Maltese Second Division. Marsa finished as champions, by just one point, and were promoted to the 2001–02 Maltese Premier League alongside Lija Athletic, who finished runners-up. Tarxien Rainbows and Żurrieq were relegated to the 2001–02 Maltese Second Division. This was the second straight relegation for Żurrieq, having been relegated from the Premier League the previous season. Marsa suffered their only defeat at the hands of Żurrieq.
The Maltese First Division 2000–01 was made up of these teams:
For a complete set of results, see 1.
| Rank | Player | Club | Goals |
|---|---|---|---|
| 1 | Kevin Loughborough | St. Patrick | 12 |
| 2 |  Johann Zammit | Mosta | 11 |
| 3 | Matthew Calascione | St. Andrews | 10 |
| 4 | Uwa Ogbodo | Qormi | 9 |
| Alan Tabone | Lija Athletic |