| Competition: | Macedonian Second League |
| Season: | 2000–01 |
| Winners: | Kumanovo |
| Promoted: | Kumanovo Napredok |
| Relegated: | Mladost GT Vardar Negotino Mesna Industrija Malesh |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The 2000–01 Macedonian Second Football League was the ninth season since its establishment. It began on 19 August 2000 and ended in May 2001.[1]