| Macedonian Football Cup | |
| Year: | 2000–01 |
| Dates: | 12 August 2000 – 24 May 2001 |
| Num Teams: | 32 |
| Defending Champions: | Sloga Jugomagnat |
| Champions: | Pelister (1st title) |
| Runner-Up: | Sloga Jugomagnat |
| Matches: | 44 |
| Goals: | 164 |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The 2000–01 Macedonian Football Cup was the 9th season of Macedonia's football knockout competition. Sloga Jugomagnat were the defending champions, having won their second title the year before after reaching their fifth Final in a row. The 2000–01 champions were Pelister who beat the defending champions to claim their first title after twice been runners-up in the first 2 Macedonian Cups in 1992–93 & 1993–94.
| Round | Date(s) | Fixtures | Clubs | New entries |
|---|---|---|---|---|
| First Round | 12, 13, 14 August & 20 September 2000 | 16 | 32 → 16 | 32 |
| Second Round | 8, 29 November 2000 | 16 | 16 → 8 | none |
| Quarter-finals | 25 February & 4 April 2001 | 8 | 8 → 4 | none |
| Semi-finals | 17 April & 9 May 2001 | 4 | 4 → 2 | none |
| Final | 24 May 2001 | 1 | 2 → 1 | none |
Matches were played on 13 August 2000.
|colspan="3" style="background-color:#97DEFF" align=center|12 August 2000|-|colspan="3" style="background-color:#97DEFF" align=center|13 August 2000|-|colspan="3" style="background-color:#97DEFF" align=center|14 August 2000|-|colspan="3" style="background-color:#97DEFF" align=center|20 September 2000|}
Source: Dnevnik.mk (archived)
The first legs were played on 8 November and second were played on 29 November 2000.
|}
The first legs were played on 25 February and second were played on 4 April 2001.
|}
The first legs were played on 17 April 2001. The second legs were originally scheduled for 3 May 2001, but were postponed to 9 May 2001, due to situation at the border with FR Yugoslavia.[1]
|}
Sloga Jugomagnat won 6–0 on aggregate.----
Pelister won 4–3 on aggregate.