| Competition: | Luxembourg National Division |
| Season: | 2000–01 |
| Winners: | F91 Dudelange (2nd titles) |
| Continentalcup1: | Champions League |
| Continentalcup1 Qualifiers: | F91 Dudelange |
| Continentalcup2: | UEFA Cup |
| Continentalcup2 Qualifiers: | Grevenmacher Etzella Ettelbruck (via cup) |
| Continentalcup3: | Intertoto Cup |
| Continentalcup3 Qualifiers: | Hobscheid |
| Relegated: | Rodange 91 Wiltz 71 |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The 2000–01 Luxembourg National Division was the 87th season of top level association football in Luxembourg.
It was performed in 12 teams, and F91 Dudelange have won the championship.