| Luxembourg Cup | |
| Year: | 2000–01 |
| Country: | Luxembourg |
| Champions: | FC Etzella Ettelbruck |
| Runner-Up: | FC Wiltz 71 |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The 2000–01 Luxembourg Cup was the 76th season of Luxembourg's annual cup competition. It began on September 2, 2000 with Round 1 and finished on May 24, 2001.
Fifty-two teams from Division 2 (IV) and Division 3 (V) entered in this round. Forty of them competed in matches, with the other twelve teams were awarded a bye. The games were played on September 3, 2008.
|colspan="3" style="background-color:#99CCCC"|2-6 September 2000Bye: US Sandweiler, FC UNA Strassen, FC Yellow Boys Weiler-la-Tour, FC Egalité Weimerskirch, AS Colmarberg, FC Marisca Mersch, FC Etoile Sportive Schouweiler, CS Bourscheid.
|colspan="3" style="background-color:#99CCCC"|7 October 2000
|colspan="3" style="background-color:#99CCCC"|22 February 2001|-|colspan="3" style="background-color:#99CCCC"|23 February 2001|-|colspan="3" style="background-color:#99CCCC"|24 February 2001|-|colspan="3" style="background-color:#99CCCC"|11 April 2001
|colspan="3" style="background-color:#99CCCC"|22 April 2001|}
|colspan="3" style="background-color:#99CCCC"|9 May 2001
|colspan="3" style="background-color:#99CCCC"|16 May 2001