| Lithuanian Football Cup | |
| Year: | 2000–01 |
| Country: | Lithuania |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The Lithuanian Football Cup 2000–01 was the 12th season of the Lithuanian annual football tournament. The competition started on 18 March 2000 with the first-round games, and ended on 19 May 2001 with the final. The defending champions were FK Ekranas.
| colspan="3" style="background:#9cc;"|18 March 2000|}
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FK Nevėžis won 3–1 on aggregate----FK Atlantas won 2–1 on aggregate----FK Žalgiris won 5–0 on aggregate----FBK Kaunas won 13–1 on aggregate
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FK Žalgiris won 6–1 on aggregate----2–2 on aggregate, FK Atlantas win on away goals