| Competition: | League of Wales |
| Season: | 2000–01 |
| Winners: | Barry Town |
| Relegated: | Inter Cardiff |
| Continentalcup1: | Champions League |
| Continentalcup1 Qualifiers: | Barry Town |
| Continentalcup2: | UEFA Cup |
| Continentalcup2 Qualifiers: | Cwmbran Town Total Network Solutions |
| Continentalcup3: | Intertoto Cup |
| Continentalcup3 Qualifiers: | Carmarthen Town |
| League Topscorer: | Graham Evans (25) |
| Matches: | 306 |
| Total Goals: | 1030 |
| Prevseason: | 1999–00 |
| Nextseason: | 2001–02 |
The 2000–01 League of Wales was the ninth season of the League of Wales since its establishment in 1992. It began on 18 August 2000 and ended on 5 May 2001.[1] The league was won by Barry Town, their fifth title.