| 2000–01 LEN Champions League | |
| League: | LEN Champions League |
| Sport: | Water Polo |
| Duration: | 2000 to 19 May 2001 |
| No Of Teams: | 8 (preliminary round) |
| Playoffs: | Final Four |
| Playoffs Link: | 2000–01 LEN Champions League#Final Four (Dubrovnik) |
| Finals Champ: | Jug Dubrovnik (2nd title) |
| Seasonslist: | LEN Champions League |
| Seasonslistnames: | Champions League |
| Prevseason Link: | 1999–2000 LEN Champions League |
| Prevseason Year: | 1999–00 |
| Nextseason Link: | 2001–02 LEN Champions League |
| Nextseason Year: | 2001–02 |
The 2000–01 LEN Champions League was the 38th edition of LEN's premier competition for men's water polo clubs. It ran from 2000 to 19 May 2001, and it was contested by 8 teams. The Final Four (semifinals, final, and third place game) took place on May 18 and May 19 in Dubrovnik.
Bazen u Gružu, Dubrovnik, Croatia
| width=25 | ! | width=220 | Team |
|---|---|---|---|
| Jug Dubrovnik | |||
| Olympiacos | |||
 Posillipo | |||
| Bečej |
| Goran Volarević, Tihomil Vranješ, Đani Pecotić, Igor Računica, Ognjen Kržić, Mile Smodlaka, Dragan Medan, Ivo Ivaniš, Alen Bošković, Andrey Belofastov, Maro Balić, Elvis Fatović, Frano Karač | |
| Head coach | |
| Veselin Đuho |