| Competition: | Iran Football's 2nd Division |
| Season: | 2000–01 |
| Nextseason: | 2001–02 |
Iran Football's 2nd Division 2000–01 season was played in three groups of nine teams each. The top two teams from each group advanced to the second round, and the top two team from that round – Aboomoslem and Malavan – gained promotion to the Iran Pro League.