| Mode: | Basketball |
| Year: | 2000–01 |
| Prev Year: | 1999–2000 |
| Next Year: | 2001–02 |
| Team: | Holy Cross Crusaders |
| Conference: | Patriot League |
| Short Conf: | Patriot |
| Record: | 22–8 |
| Conf Record: | 10–2 |
| Head Coach: | Ralph Willard |
| Hc Year: | 2nd |
| Stadium: | Hart Center |
| Champion: | Patriot League regular season and tournament champions |
| Tourney: | NCAA tournament |
| Tourney Result: | First round |
The 2000–01 Holy Cross Crusaders men's basketball team represented the College of the Holy Cross during the 2000–01 NCAA Division I men's basketball season. The Crusaders, led by 2nd-year head coach Ralph Willard, played their home games at the Hart Center and were members of the Patriot League. They finished the season 22–8, 10–2 in Patriot League play to win the regular season league title. As the #1 seed, they defeated Lehigh and Navy to be champions of the Patriot League tournament and earn the conference's automatic bid to the NCAA tournament. Playing as the No. 14 seed in the East region, they were beaten by No. 3 seed Kentucky, 72–68.[1]
|-!colspan=9 style=|Non-conference regular season
|-!colspan=9 style=|Patriot League regular season
|-!colspan=9 style=| Patriot League Tournament|-!colspan=9 style=|NCAA Tournament[3] [4] [5]