| Competition: | Highland League |
| Season: | 2000–01 |
| Winners: | Cove Rangers |
| Total Goals: | 622 |
| Average Goals: | 3.42 |
| Prevseason: | 1999–00 |
| Nextseason: | 2001–02 |
The 2000–01 Highland Football League was won by Cove Rangers. Fort William finished bottom. Elgin City & Peterhead left the Highland League to join the Scottish Football League, reducing the number of teams from 16 to 14 this season.