The 2000–01 Hellenic Football League season was the 48th in the history of the Hellenic Football League, a football competition in England.
At the end of the previous season the Hellenic League merged with the Chiltonian League. 17 clubs from the latter formed Division One East, while Hellenic League Division One clubs formed Division One West.
| Competition: | Hellenic Football League Premier Division |
| Season: | 2000–01 |
| Winners: | Swindon Supermarine |
| Promoted: | Swindon Supermarine |
| Relegated: | Milton United |
| Matches: | 380 |
| Total Goals: | 1216 |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The Premier Division featured 17 clubs which competed in the division last season, along with three new clubs:
| Competition: | Hellenic Football League Division One East |
| Season: | 2000–01 |
| Winners: | Henley Town |
| Promoted: | Henley Town Southall Town |
| Matches: | 272 |
| Total Goals: | 1079 |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
Division One East was formed by 17 clubs from the Chiltonian League, adsorbed by the Hellenic League at the end of the previous season.
| Competition: | Hellenic Football League Division One West |
| Season: | 2000–01 |
| Winners: | Gloucester United |
| Promoted: | Gloucester United Bishop's Cleeve |
| Matches: | 240 |
| Total Goals: | 869 |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
Division One West featured 13 clubs which competed in Division One last season, along with three new clubs: