| Mode: | Basketball |
| Sex: | none |
| Year: | 2000–01 |
| Team: | Hampton Pirates |
| Conference: | Mid-Eastern Athletic Conference |
| Short Conf: | MEAC |
| Record: | 25–7 |
| Conf Record: | 14–4 |
| Hc Year: | 4th |
| Champion: | MEAC regular season co-champions and tournament champions |
| Tourney Result: | second round |
The 2000–01 Hampton Pirates men's basketball team represented Hampton University during the 2000–01 NCAA Division I men's basketball season. The Pirates were members of the Mid-Eastern Athletic Conference and were coached by Steve Merfeld, his fourth year as head coach. The Pirates played home games at the Hampton Convocation Center.
Hampton finished the season with a 25–7 record and an 14–4 MEAC record. The season was highlighted by the Pirates winning their first ever game in the NCAA Division I men's basketball tournament, becoming the fourth 15-seed to beat a 2-seed in the tournament. Hampton defeated Iowa State[1] before losing to Georgetown[2] in the second round.
|-!colspan=9 style=| Regular season|-!colspan=9 style=| MEAC tournament|-!colspan=9 style=| NCAA tournament|-