| Season: | 2000–01 |
| Dates: | 18 August 2000 – 31 May 2001 |
| Winners: | PSV (16th title) |
| League Topscorer: | Mateja Kežman (24 goals)[1] |
| Total Goals: | 922 |
| Average Goals: | 3.01 |
| Nextseason: | 2001–02 |
The 2000–01 Eredivisie season was contested by 18 teams. PSV won the championship.
In the promotion/relegation competition, eight entrants (six from the Eerste Divisie and two from this league) entered in two groups. The group winners were promoted to (or remained in) the Eredivisie.