The 2000–01 season was the 59th in the history of Eastern Counties Football League a football competition in England.[1]
AFC Sudbury were champions, winning their first Eastern Counties Football League title after the new club was formed in 1999.
| Competition: | Eastern Counties Football League Premier Division |
| Season: | 2000–01 |
| Winners: | AFC Sudbury |
| Relegated: | Halstead Town Warboys Town |
| Matches: | 462 |
| Total Goals: | 1471 |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The Premier Division featured 20 clubs which competed in the division last season, along with two new clubs, promoted from Division One:
Also, Felixstowe Port & Town merged with Walton United to form new club Felixstowe & Walton United.
| Competition: | Eastern Counties Football League Division One |
| Season: | 2000–01 |
| Winners: | Swaffham Town |
| Promoted: | Dereham Town Swaffham Town |
| Matches: | 272 |
| Total Goals: | 948 |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
Division One featured 16 clubs which competed in the division last season, along with one new club: