The 2000–01 Combined Counties Football League season was the 23rd in the history of the Combined Counties Football League, a football competition in England.
| Competition: | Combined Counties Football League |
| Season: | 2000–01 |
| Winners: | Cove |
| Matches: | 419 |
| Total Goals: | 1629 |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The league featured 20 clubs from the previous season, along with one new club: