| Competition: | Campionato Sammarinese di Calcio |
| Season: | 2000–01 |
| Winners: | S.S. Cosmos |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The 2000–01 Campionato Sammarinese di Calcio season was the 16th season since its establishment. It was contested by 15 teams, and S.S. Cosmos won the championship.
All teams play twice against the teams within their own group and once against the teams from the other group.