| Competition: | Beta Ethniki |
| Season: | 2000–01 |
| Winners: | Egaleo |
| Promoted: | Egaleo Akratitos |
| Relegated: | Nafpaktiakos Asteras Panetolikos Kavala Trikala AEL Panelefsiniakos |
| Matches: | 306 |
| Total Goals: | 653 |
| Biggest Home Win: | PAS Giannina 5–0 Veria PAS Giannina 5–0 Diagoras |
| Biggest Away Win: | Agrotikos Asteras 1–4 Kallithea |
| Highest Scoring: | Eth. Asteras 4–3 Diagoras Ethnikos 4–3 Kallithea --> |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
Beta Ethniki 2000–01 complete season.
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| Rank | Player | Club | Goals |
|---|---|---|---|
| 1 | Thomas Makris | Egaleo | 16 |
| 2 | Georgios Zacharopoulos | Kallithea | 15 |
| 3 | Lefteris Velentzas | Akratitos | 13 |
| Georgios Vlachoudis | Olympiacos Volos | ||
| Christos Karapitsos | Panegialios | ||
| 6 | Stavros Labriakos | Apollon Athens | 12 |
| Karim Mouzaoui | Apollon Kalamarias | ||
| Dimitris Papadopoulos | Akratitos | ||
| 9 | Anestis Agritis | Egaleo | 11 |
| Christoforos Andriadakis | Agios Nikolaos | ||