| Club: | Ayr United |
| Season: | 2000–01 |
| Manager: | Gordon Dalziel |
| Chairman: | Bill Barr |
| Stadium: | Somerset Park |
| League: | Scottish First Division |
| League Result: | Runners-Up |
| Cup1: | Scottish Cup |
| Cup1 Result: | Third Round, lost to Inverness CT |
| Cup2: | League Cup |
| Cup2 Result: | First Round, lost to Dumbarton |
| Cup3: | Scottish Challenge Cup |
| Cup3 Result: | First Round, lost to Brechin City |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
| Pattern B1: | _blackshoulders |
| Pattern La1: | _blackshoulders |
| Pattern Ra1: | _blackshoulders |
| Pattern Sh1: | _black_stripe |
| Pattern So1: | _band_black |
| Body1: | FFFFFF |
| Leftarm1: | FFFFFF |
| Rightarm1: | FFFFFF |
| Shorts1: | FFFFFF |
| Socks1: | FFFFFF |
| Pattern B2: | _whiteshoulders |
| Pattern La2: | _whiteshoulders |
| Pattern Ra2: | _whiteshoulders |
| Pattern Sh2: | _white_stripes |
| Pattern So2: | _band_black |
| Body2: | 000000 |
| Leftarm2: | 000000 |
| Rightarm2: | 000000 |
| Shorts2: | 000000 |
| Socks2: | 000000 |
The 2000–01 season is the 91st season of competitive football by Ayr United.
See main article: 2000–01 Scottish First Division.
See main article: 2000-01 Scottish League Cup.
See main article: 2000-01 Scottish Challenge Cup.
See main article: 2000-01 Scottish Cup.