| Club: | Atalanta |
| Season: | 2000–01 |
| Manager: | Giovanni Vavassori |
| Chairman: | Ivan Ruggeri |
| Stadium: | Stadio Atleti Azzurri d'Italia |
| League: | Serie A |
| League Result: | 7th |
| Cup1: | Coppa Italia |
| Cup1 Result: | Quarter-finals |
| League Topscorer: | Nicola Ventola (10) |
| Season Topscorer: | Nicola Ventola (11) |
| Average Attendance: | 18,663[1] |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
Atalanta B.C. returned to Serie A following a two-year absence, and immediately established itself on the top half of the table, finishing in 7th place. Coach Giovanni Vavassori was hailed as the main contributor to the success, having come from the youth side a couple of years before, first promoting the club, and then having such a successful inaugural Serie A campaign. Inter-owned Nicola Ventola scored ten league goals, enough to persuade Inter to take him back, and playmaker Cristiano Doni got his proper breakthrough as well. Experienced Massimo Carrera held the defence together, and was also widely praised, while goalkeeper Ivan Pelizzoli was signed by champions Roma.
See main article: 2000–01 Serie A.
See main article: 2000–01 Coppa Italia.
|-! colspan=10 style=background:#DCDCDC; text-align:center| Players transferred out during the season
| width=15 | width=15 | width=15 | width=15 | width=145 | Name | width=130 | Serie A | width=130 | Coppa Italia | width=130 | Total | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 10 | FW |  | Nicola Ventola | 10 | 1 | 11 | ||||||||
| 2 | 27 | MF | | Cristiano Doni | 7 | 3 | 10 | ||||||||
| 3 | 11 | FW | | Maurizio Ganz | 5 | 4 | 9 | ||||||||
| 4 | 9 | FW | | Fausto Rossini | 4 | 1 | 5 | ||||||||
| 3 | MF | | Domenico Morfeo | 5 | 0 | 5 | |||||||||
| 6 | 7 | FW | | Marco Nappi | 1 | 2 | 3 | ||||||||
| 7 | 26 | DF | | Cristian Zenoni | 1 | 1 | 2 | ||||||||
| 30 | DF | | Gianpaolo Bellini | 0 | 2 | 2 | |||||||||
| 8 | DF | | Luciano Zauri | 1 | 1 | 2 | |||||||||
| 29 | MF | | Massimo Donati | 1 | 1 | 2 | |||||||||
| 11 | 15 | DF | | Stefano Lorenzi | 1 | 0 | 1 | ||||||||
| 21 | MF | | Alex Pinardi | 0 | 1 | 1 | |||||||||
| 19 | DF | | Damiano Zenoni | 1 | 0 | 1 | |||||||||
| Totals | 37 | 17 | 54 | ||||||||||||