| Competition: | 2. Bundesliga |
| Season: | 2000–01 |
| Winners: | 1. FC Nürnberg |
| Promoted: | 1. FC Nürnberg Borussia Mönchengladbach FC St. Pauli |
| Relegated: | VfL Osnabrück SSV Ulm 1846 Stuttgarter Kickers Chemnitzer FC |
| League Topscorer: | Artur Wichniarek Olivier Djappa (18) |
| Prevseason: | 1999–2000 |
| Nextseason: | 2001–02 |
The 2000–01 2. Bundesliga was the 27th season of the 2. Bundesliga, the second tier of the German football league system. 1. FC Nürnberg, Borussia Mönchengladbach and FC St. Pauli were promoted to the Bundesliga while VfL Osnabrück, SSV Ulm 1846, Stuttgarter Kickers and Chemnitzer FC were relegated to the Regionalliga.
For the 2000–01 season LR Ahlen, SSV Reutlingen, 1. FC Saarbrücken and VfL Osnabrück were newly promoted to the 2. Bundesliga from the Regionalliga while SSV Ulm 1846, Arminia Bielefeld and MSV Duisburg had been relegated to the league from the Bundesliga.[1]
The league's top scorers:[2]