2-oxopropyl-CoM reductase (carboxylating) explained

2-oxopropyl-CoM reductase (carboxylating)
Ec Number:	1.8.1.5
Go Code:	0050628

In enzymology, a 2-oxopropyl-CoM reductase (carboxylating) is an enzyme that catalyzes the chemical reaction

2-mercaptoethanesulfonate + acetoacetate + NADP⁺

\rightleftharpoons 2-(2-oxopropylthio)ethanesulfonate + CO₂ + NADPH

The 3 substrates of this enzyme are 2-mercaptoethanesulfonate, acetoacetate, and NADP⁺, whereas its 3 products are 2-(2-oxopropylthio)ethanesulfonate, CO₂, and NADPH.

This enzyme belongs to the family of oxidoreductases, specifically those acting on a sulfur group of donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 2-mercaptoethanesulfonate, acetoacetate:NADP+ oxidoreductase (decarboxylating). Other names in common use include NADPH:2-(2-ketopropylthio)ethanesulfonate, oxidoreductase/carboxylase, and NADPH:2-ketopropyl-coenzyme M oxidoreductase/carboxylase.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes and .

References

• Allen JR, Clark DD, Krum JG, Ensign SA . 1999 . A role for coenzyme M (2-mercaptoethanesulfonic acid) in a bacterial pathway of aliphatic epoxide carboxylation . Proc. Natl. Acad. Sci. U.S.A. . 96 . 8432 - 7 . 10411892 . 10.1073/pnas.96.15.8432 . 15 . 17533 . 1999PNAS...96.8432A . free .
• Clark DD, Allen JR, Ensign SA . 2000 . Characterization of five catalytic activities associated with the NADPH:2-ketopropyl-coenzyme M [2-(2-ketopropylthio)ethanesulfonate] oxidoreductase/carboxylase of the Xanthobacter strain Py2 epoxide carboxylase system . Biochemistry . 39 . 1294 - 304 . 10684609 . 10.1021/bi992282p . 6 .