2-oxoglutarate/L-arginine monooxygenase/decarboxylase (succinate-forming) explained

2-oxoglutarate/L-arginine monooxygenase/decarboxylase (succinate-forming)
Ec Number:	1.14.11.34

2-oxoglutarate/L-arginine monooxygenase/decarboxylase (succinate-forming) (ethylene-forming enzyme, EFE) is an enzyme with systematic name L-arginine,2-oxoglutarate:oxygen oxidoreductase (succinate-forming).^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

2-oxoglutarate + L-arginine + O₂

\rightleftharpoons succinate + CO₂ + guanidine + (S)-1-pyrroline-5-carboxylate + H₂O (overall reaction)

(1a) 2-oxoglutarate + L-arginine + O₂

\rightleftharpoons succinate + CO₂ + L-hydroxyarginine

(1b) L-hydroxyarginine

\rightleftharpoons guanidine + (S)-1-pyrroline-5-carboxylate + H₂O

2-oxoglutarate/L-arginine monooxygenase/decarboxylase catalyses two cycles of the ethylene-forming reaction.

Notes and References

1. Nagahama K, Ogawa T, Fujii T, Tazaki M, Tanase S, Morino Y, Fukuda H . Purification and properties of an ethylene-forming enzyme from Pseudomonas syringae pv. phaseolicola PK2 . Journal of General Microbiology . 137 . 10 . 2281–6 . October 1991 . 1770346 . 10.1099/00221287-137-10-2281 . free .
2. Fukuda H, Ogawa T, Tazaki M, Nagahama K, Fujii T, Tanase S, Morino Y . Two reactions are simultaneously catalyzed by a single enzyme: the arginine-dependent simultaneous formation of two products, ethylene and succinate, from 2-oxoglutarate by an enzyme from Pseudomonas syringae . Biochemical and Biophysical Research Communications . 188 . 2 . 483–9 . October 1992 . 1445291 . 10.1016/0006-291X(92)91081-Z .
3. Fukuda H, Ogawa T, Ishihara K, Fujii T, Nagahama K, Omata T, Inoue Y, Tanase S, Morino Y . Molecular cloning in Escherichia coli, expression, and nucleotide sequence of the gene for the ethylene-forming enzyme of Pseudomonas syringae pv. phaseolicola PK2 . Biochemical and Biophysical Research Communications . 188 . 2 . 826–32 . October 1992 . 1445325 . 10.1016/0006-291X(92)91131-9 .