2-methyl-branched-chain-enoyl-CoA reductase explained

2-methyl-branched-chain-enoyl-CoA reductase
Ec Number:	1.3.8.5
Cas Number:	122320-06-3
Go Code:	0047119

In enzymology, a 2-methyl-branched-chain-enoyl-CoA reductase is an enzyme that catalyzes the chemical reaction

2-methylbutanoyl-CoA + electron transfer flavoprotein

\rightleftharpoons 2-methylcrotonoyl-CoA + reduced electron transfer flavoprotein + H⁺

Thus, the two substrates of this enzyme are 2-methylbutanoyl-CoA and an electron transfer flavoprotein, whereas its 3 products are 2-methylcrotonoyl-CoA, reduced electron transfer flavoprotein, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-CH group of donors with flavin as acceptor. The systematic name of this enzyme class is 2-methyl-branched-chain-acyl-CoA:electron-transfer flavoprotein 2-oxidoreductase . This enzyme participates in the degradation of isoleucine. It employs one cofactor, FAD.

References

• Komuniecki R, Fekete S, Thissen-Parra J . 1985 . Purification and characterization of the 2-methyl branched-chain Acyl-CoA dehydrogenase, an enzyme involved in NADH-dependent enoyl-CoA reduction in anaerobic mitochondria of the nematode, Ascaris suum . J. Biol. Chem. . 260 . 4770 - 7 . 3988734 . 8 .
• Komuniecki R, McCrury J, Thissen J, Rubin N . 1989 . Electron-transfer flavoprotein from anaerobic Ascaris suum mitochondria and its role in NADH-dependent 2-methyl branched-chain enoyl-CoA reduction . Biochim. Biophys. Acta . 975 . 127 - 31 . 2736251 . 1 . 10.1016/s0005-2728(89)80210-5.