2-hydroxy-6-oxonona-2,4-dienedioate hydrolase explained

2-hydroxy-6-oxonona-2,4-dienedioate hydrolase
Ec Number:	3.7.1.14

2-hydroxy-6-oxonona-2,4-dienedioate hydrolase (mhpC (gene)) is an enzyme with systematic name (2Z,4E)-2-hydroxy-6-oxona-2,4-dienedioate succinylhydrolase.^{[1] [2] [3] [4] [5] [6]} This enzyme catalyses the following chemical reaction:

1. (2Z,4E)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate + H₂O

\rightleftharpoons (2Z)-2-hydroxypenta-2,4-dienoate + succinate

1. (2Z,4E,7E)-2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate + H₂O

\rightleftharpoons (2Z)-2-hydroxypenta-2,4-dienoate + fumarate

This enzyme catalyses a step in a pathway of phenylpropanoid compounds degradation.

Notes and References

1. Burlingame R, Chapman PJ . Catabolism of phenylpropionic acid and its 3-hydroxy derivative by Escherichia coli . Journal of Bacteriology . 155 . 1 . 113–21 . July 1983 . 6345502 . 217659 .
2. Burlingame RP, Wyman L, Chapman PJ . Isolation and characterization of Escherichia coli mutants defective for phenylpropionate degradation . Journal of Bacteriology . 168 . 1 . 55–64 . October 1986 . 3531186 . 213419 .
3. Chemistry of extradiol aromatic ring cleavage: isolation of a stable dienol ring fission intermediate and stereochemistry of its enzymatic hydrolytic cleavage . Lam WW, Bugg TD . J. Chem. Soc., Chem. Commun. . 1994 . 10 . 1163–1164 . 10.1039/c39940001163 . 10 .
4. Lam WW, Bugg TD . Purification, characterization, and stereochemical analysis of a C-C hydrolase: 2-hydroxy-6-keto-nona-2,4-diene-1,9-dioic acid 5,6-hydrolase . Biochemistry . 36 . 40 . 12242–51 . October 1997 . 9315862 . 10.1021/bi971115r .
5. Ferrández A, Garciá JL, Díaz E . Genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl)propionate catabolic pathway of Escherichia coli K-12 . Journal of Bacteriology . 179 . 8 . 2573–81 . April 1997 . 9098055 . 179006 .
6. Díaz E, Ferrández A, García JL . Characterization of the hca cluster encoding the dioxygenolytic pathway for initial catabolism of 3-phenylpropionic acid in Escherichia coli K-12 . Journal of Bacteriology . 180 . 11 . 2915–23 . June 1998 . 9603882 . 107259 .