2-hydroxy-4-carboxymuconate semialdehyde hemiacetal dehydrogenase explained

2-hydroxy-4-carboxymuconate semialdehyde hemiacetal dehydrogenase
Ec Number:	1.1.1.312

2-hydroxy-4-carboxymuconate semialdehyde hemiacetal dehydrogenase (2-hydroxy-4-carboxymuconate 6-semialdehyde dehydrogenase, 4-carboxy-2-hydroxy-cis,cis-muconate-6-semialdehyde:NADP⁺ oxidoreductase, alpha-hydroxy-gamma-carboxymuconic epsilon-semialdehyde dehydrogenase, 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase, LigC, ProD) is an enzyme with systematic name 4-carboxy-2-hydroxymuconate semialdehyde hemiacetal:NADP⁺ 2-oxidoreductase.^{[1] [2] [3] [4]} This enzyme catalyses the following chemical reaction

4-carboxy-2-hydroxymuconate semialdehyde hemiacetal + NADP⁺

\rightleftharpoons 2-oxo-2H-pyran-4,6-dicarboxylate + NADPH + H⁺

Unsubstituted aliphatic or aromatic aldehydes or glucose cannot serve as substrate of this enzyme.

Notes and References

1. Maruyama K, Ariga N, Tsuda M, Deguchi K . Purification and properties of alpha-hydroxy-gamma-carboxymuconic epsilon-semialdehyde dehydrogenase . Journal of Biochemistry . 83 . 4 . 1125–34 . April 1978 . 26671 .
2. Maruyama K . Isolation and identification of the reaction product of alpha-hydroxy-gamma-carboxymuconic epsilon-semialdehyde dehydrogenase . Journal of Biochemistry . 86 . 6 . 1671–7 . December 1979 . 528534 .
3. Maruyama K . Purification and properties of 2-pyrone-4,6-dicarboxylate hydrolase . Journal of Biochemistry . 93 . 2 . 557–65 . February 1983 . 6841353 .
4. Masai E, Momose K, Hara H, Nishikawa S, Katayama Y, Fukuda M . Genetic and biochemical characterization of 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase and its role in the protocatechuate 4,5-cleavage pathway in Sphingomonas paucimobilis SYK-6 . Journal of Bacteriology . 182 . 23 . 6651–8 . December 2000 . 11073908 . 111406 . 10.1128/jb.182.23.6651-6658.2000 .