2-haloacid dehalogenase (configuration-inverting) explained

2-haloacid dehalogenase (configuration-inverting)
Ec Number:	3.8.1.10

2-haloacid dehalogenase (configuration-inverting) (2-haloalkanoic acid dehalogenase, 2-haloalkanoid acid halidohydrolase, DL-2-haloacid dehalogenase, DL-2-haloacid dehalogenase (inversion of configuration), DL-2-haloacid halidohydrolase (inversion of configuration), DL-DEXi, (R,S)-2-haloacid dehalogenase (configuration-inverting)) is an enzyme with systematic name (S)-2-haloacid dehalogenase (configuration-inverting).^{[1] [2] [3] [4] [5] [6] [7] [8] [9]} This enzyme catalyses the following chemical reaction

(1) (S)-2-haloacid + H₂O

\rightleftharpoons (R)-2-hydroxyacid + halide

(2) (R)-2-haloacid + H₂O

\rightleftharpoons (S)-2-hydroxyacid + halide

This enzyme dehalogenates both (S)- and (R)-2-haloalkanoic acids.

Notes and References

1. Motosugi K, Esaki N, Soda K . Bacterial assimilation of D- and L-2-chloropropionates and occurrence of a new dehalogenase . Archives of Microbiology . 131 . 3 . 179–83 . May 1982 . 7103659 . 10.1007/BF00405875 .
2. Motosugi K, Esaki N, Soda K . Purification and properties of a new enzyme, DL-2-haloacid dehalogenase, from Pseudomonas sp . Journal of Bacteriology . 150 . 2 . 522–7 . May 1982 . 7068529 . 216397 .
3. Motosugi K, Esaki N, Soda K . Enzymatic preparation of D- and L-lactic acid from racemic 2-chloropropionic acid . Biotechnology and Bioengineering . 26 . 7 . 805–6 . July 1984 . 18553452 . 10.1002/bit.260260729 .
4. Bacterial 2-haloacid dehalogenases: structures and reaction mechanisms . Kurihara T, Esaki N, Soda K . J. Mol. Catal., B Enzym. . 2000 . 10 . 1–3 . 57–65 . 10.1016/S1381-1177(00)00108-9.
5. Liu JQ, Kurihara T, Hasan AK, Nardi-Dei V, Koshikawa H, Esaki N, Soda K . Purification and characterization of thermostable and nonthermostable 2-haloacid dehalogenases with different stereospecificities from Pseudomonas sp. strain YL . Applied and Environmental Microbiology . 60 . 7 . 2389–93 . July 1994 . 8074519 . 201661 .
6. Cairns SS, Cornish A, Cooper RA . Cloning, sequencing and expression in Escherichia coli of two Rhizobium sp. genes encoding haloalkanoate dehalogenases of opposite stereospecificity . European Journal of Biochemistry . 235 . 3 . 744–9 . February 1996 . 8654424 . 10.1111/j.1432-1033.1996.t01-1-00744.x . free .
7. Partial purification, stereospecificity and stoichiometry of three dehalogenases from a Rhizobium species . Leigh JA, Skinner AJ, Cooper RA . FEMS Microbiol. Lett. . 1988 . 49 . 3 . 353–356 . 10.1111/j.1574-6968.1988.tb02756.x. free .
8. Weightman AJ, Weightman AL, Slater JH . Stereospecificity of 2-monochloropropionate dehalogenation by the two dehalogenases of Pseudomonas putida PP3: evidence for two different dehalogenation mechanisms . Journal of General Microbiology . 128 . 8 . 1755–62 . August 1982 . 7142958 . 10.1099/00221287-128-8-1755 . free .
9. Bacterial 2-haloacid dehalogenases: Structures and catalytic properties . Soda K, Kurihara T, Liu, JQ, Nardi-Dei V, Park C, Miyagi M, Tsunasawa S, Esaki N . Pure Appl. Chem. . 1996 . 68 . 11 . 2097–2103 . 10.1351/pac199668112097. free .