2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase explained

2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Ec Number:	4.6.1.12
Go Code:	0008685

2-C-Methyl-D-erythritol 2,4-cyclodiphosphate synthase (MEcPP synthase, IspF, EC 4.6.1.12) is a zinc-dependent enzyme and a member of the YgbB N terminal protein domain, which participates in the MEP pathway (non-mevalonate pathway) of isoprenoid precursor biosynthesis. It catalyzes the following reaction:^[1]

4-diphosphocytidyl-2-C-methyl-D)erythritol 2-phosphate

\rightleftharpoons 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP

The enzyme is considered a phosphorus-oxygen lyase. The systematic name of this enzyme class is 2-phospho-4-(cytidine 5′-diphospho)-2-C-methyl-D-D-erythritol CMP-lyase (cyclizing; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate-forming). Other names in common use include IspF, YgbB and MEcPP synthase.

Structural studies

As of late 2007, 20 structures have been solved for this class of enzymes, with PDB accession codes,,,,,,,,,,,,,,,,,,, and .

References

1. Biosynthesis of terpenoids: YgbB protein converts 4-diphosphocytidyl-2C-methyl-d-erythritol 2-phosphate to 2C-methyl-d-erythritol 2,4-cyclodiphosphate. Herz S, Wungsintaweekul J, Schuhr CA, Hecht S, Lüttgen H, Sagner S, Fellermeier M, Eisenreich W, Zenk MH, Bacher A, Rohdich F. 2000. Proc. Natl. Acad. Sci. USA . 6 . 10.1073/pnas.040554697. 10694574. 15955. 97. 2486–90. 2000PNAS...97.2486H. free.