2-Aminopurine Explained

2-Aminopurine, a purine analog of guanine and adenine, is a fluorescent molecular marker used in nucleic acid research.^[1] It most commonly pairs with thymine as an adenine-analogue, but can also pair with cytosine as a guanine-analogue.^[2] For this reason it is sometimes used in the laboratory for mutagenesis.

See also

• Nucleic acid analogues

Notes and References

1. Jean JM, Hall KB . 2-Aminopurine fluorescence quenching and lifetimes: role of base stacking . Proc. Natl. Acad. Sci. U.S.A. . 98 . 1 . 37–41 . 2001 . 11120885 . 10.1073/pnas.011442198 . 14540. free .
2. Sowers LC, Fazakerley GV, Eritja R, Kaplan BE, Goodman MF . Base pairing and mutagenesis: observation of a protonated base pair between 2-aminopurine and cytosine in an oligonucleotide by proton NMR . Proc. Natl. Acad. Sci. U.S.A. . 83 . 5434–5438. 1986 . 10.1073/pnas.83.15.5434 . 3461441 . 15 . 386301. 1986PNAS...83.5434S . free .