2,5-didehydrogluconate reductase explained

2,5-didehydrogluconate reductase
Ec Number:	1.1.1.274
Cas Number:	95725-95-4
Go Code:	0050580

In enzymology, a 2,5-didehydrogluconate reductase is an enzyme that catalyzes the chemical reaction

2-dehydro-D-gluconate + NADP⁺

\rightleftharpoons 2,5-didehydro-D-gluconate + NADPH + H⁺

Thus, the two substrates of this enzyme are 2-dehydro-D-gluconate and NADP⁺, whereas its 3 products are 2,5-didehydro-D-gluconate, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD⁺ or NADP⁺ as acceptor. The systematic name of this enzyme class is 2-dehydro-D-gluconate:NADP⁺ 2-oxidoreductase. Other names in common use include 2,5-diketo-D-gluconate reductase, and YqhE reductase.

Structural studies

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code .

References

• Yum DY, Lee BY, Pan JG . 1999 . Identification of the yqhE and yafB genes encoding two 2, 5-diketo-D-gluconate reductases in Escherichia coli . Appl. Environ. Microbiol. . 65 . 3341 - 6 . 10427017 . 8 . 10.1128/AEM.65.8.3341-3346.1999 . 91502 .
• Yum DY, Lee BY, Hahm DH, Pan JG . 1998 . The yiaE gene, located at 80.1 minutes on the Escherichia coli chromosome, encodes a 2-ketoaldonate reductase . J. Bacteriol. . 180 . 5984 - 8 . 9811658 . 22 . 10.1128/JB.180.22.5984-5988.1998 . 107674 .
• Habrych M, Rodriguez S, Stewart JD . 2002 . Purification and identification of an Escherichia coli beta-keto ester reductase as 2,5-diketo-D-gluconate reductase YqhE . Biotechnol. Prog. . 18 . 257 - 61 . 11934293 . 10.1021/bp0101841 . 2 . 23141855 .