2,4-Dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2 -D-glucosyltransferase explained

2,4-Dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucosyltransferase
Ec Number:2.4.1.202
Cas Number:122544-56-3

2,4-Dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucosyltransferase (uridine diphosphoglucose-2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-glucosyltransferase, BX8, BX9, benzoxazinoid glucosyltransferase, DIMBOA glucosyltransferase) is an enzyme with systematic name UDP-alpha-D-glucose:2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-beta-D-glucosyltransferase.[1] [2] This enzyme catalyses the following chemical reaction

(1) UDP-alpha-D-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one

\rightleftharpoons

UDP + (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside

(2) UDP-alpha-D-glucose + 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one

\rightleftharpoons

UDP + (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside

The enzyme is involved in the detoxification of the benzoxazinoids, DIBOA (2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one) and DIMBOA (2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one).

Notes and References

  1. Bailey BA, Larson RL . Hydroxamic Acid glucosyltransferases from maize seedlings . Plant Physiology . 90 . 3 . 1071–6 . July 1989 . 16666853 . 1061845 . 10.1104/pp.90.3.1071 .
  2. von Rad U, Hüttl R, Lottspeich F, Gierl A, Frey M . Two glucosyltransferases are involved in detoxification of benzoxazinoids in maize . The Plant Journal . 28 . 6 . 633–42 . December 2001 . 11851909 . 10.1046/j.1365-313x.2001.01161.x . free .