2'-phosphotransferase explained

2'-phosphotransferase
Ec Number:	2.7.1.160

In enzymology, a 2'-phosphotransferase is an enzyme that catalyzes the chemical reaction

2'-phospho-[ligated tRNA] + NAD⁺

\rightleftharpoons mature tRNA + ADP-ribose 1,2-phosphate + nicotinamide + H₂O

Thus, the two substrates of this enzyme are 2'-phospho-[ligated tRNA] and NAD⁺, whereas its 4 products are mature tRNA, ADP-ribose 1,2-phosphate, nicotinamide, and H₂O.

This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is 2'-phospho-[ligated tRNA]:NAD+ phosphotransferase. Other names in common use include yeast 2'-phosphotransferase, Tpt1, Tpt1p, and 2'-phospho-tRNA:NAD+ phosphotransferase.

References

• Steiger MA, Kierzek R, Turner DH, Phizicky EM . 2001 . Substrate recognition by a yeast 2'-phosphotransferase involved in tRNA splicing and by its Escherichia coli homolog . Biochemistry . 40 . 14098 - 105 . 11705403 . 10.1021/bi011388t . 46 .
• Spinelli SL, Kierzek R, Turner DH, Phizicky EM . 1999 . Transient ADP-ribosylation of a 2'-phosphate implicated in its removal from ligated tRNA during splicing in yeast . J. Biol. Chem. . 274 . 2637 - 44 . 9915792 . 10.1074/jbc.274.5.2637 . 5 . free .
• Culver GM, McCraith SM, Consaul SA, Stanford DR, Phizicky EM . 1997 . A 2'-phosphotransferase implicated in tRNA splicing is essential in Saccharomyces cerevisiae . J. Biol. Chem. . 272 . 13203 - 10 . 9148937 . 10.1074/jbc.272.20.13203 . 20 . free .
• McCraith SM, Phizicky EM . 1991 . An enzyme from Saccharomyces cerevisiae uses NAD+ to transfer the splice junction 2'-phosphate from ligated tRNA to an acceptor molecule . J. Biol. Chem. . 266 . 11986 - 92 . 2050693 . 18 .
• Hu QD, Lu H, Huo K, Ying K, Li J, Xie Y, Mao Y, Li YY . 2003 . A human homolog of the yeast gene encoding tRNA 2'-phosphotransferase: cloning, characterization and complementation analysis . Cell. Mol. Life Sci. . 60 . 1725 - 32 . 14504659 . 10.1007/s00018-003-3107-7 . 8 . 11138648 .
• Steiger MA, Jackman JE, Phizicky EM . 2005 . Analysis of 2'-phosphotransferase (Tpt1p) from Saccharomyces cerevisiae: evidence for a conserved two-step reaction mechanism . RNA . 11 . 99 - 106 . 15611300 . 10.1261/rna.7194605 . 1 . 1370695 .
• Sawaya R, Schwer B, Shuman S . 2005 . Structure-function analysis of the yeast NAD+-dependent tRNA 2'-phosphotransferase Tpt1 . RNA . 11 . 107 - 13 . 15611301 . 10.1261/rna.7193705 . 1 . 1370696 .
• Kato-Murayama M, Bessho Y, Shirouzu M, Yokoyama S . 2005 . Crystal structure of the RNA 2'-phosphotransferase from Aeropyrum pernix K1 . J. Mol. Biol. . 348 . 295 - 305 . 15811369 . 10.1016/j.jmb.2005.02.049 . 2 .