2'-hydroxybiphenyl-2-sulfinate desulfinase explained

2-(2-hydroxyphenyl)benzenesulfinate hydrolase
Ec Number:	3.13.1.3
Go Code:	0018740

In enzymology, a 2'-hydroxybiphenyl-2-sulfinate desulfinase is an enzyme that catalyzes the chemical reaction

2'-hydroxybiphenyl-2-sulfinate + H₂O

\rightleftharpoons

2-hydroxybiphenyl + sulfite

Thus, the two substrates of this enzyme are 2'-hydroxybiphenyl-2-sulfinate and H₂O, whereas its two products are 2-hydroxybiphenyl and sulfite.

This enzyme belongs to the family of hydrolases, specifically those acting on carbon-sulfur bonds. The systematic name of this enzyme class is 2'-hydroxybiphenyl-2-sulfinate sulfohydrolase. Other names in common use include gene dszB-encoded hydrolase, 2-(2-hydroxyphenyl) benzenesulfinate:H₂O hydrolase, DszB, HBPSi desulfinase, 2-(2-hydroxyphenyl) benzenesulfinate sulfohydrolase, HPBS desulfinase, 2-(2-hydroxyphenyl)benzenesulfinate hydrolase, 2-(2'-hydroxyphenyl)benzenesulfinate desulfinase, and 2-(2-hydroxyphenyl)benzenesulfinate desulfinase.

Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes,, and .

References

Oldfield C, Pogrebinsky O, Simmonds J, Olson ES, Kulpa CF . Elucidation of the metabolic pathway for dibenzothiophene desulphurization by Rhodococcus sp. strain IGTS8 (ATCC 53968) . Microbiology . 143 . 9 . 2961–73 . September 1997 . 9308179 . 10.1099/00221287-143-9-2961 . free .
Nakayama N, Matsubara T, Ohshiro T, Moroto Y, Kawata Y, Koizumi K, Hirakawa Y, Suzuki M, Maruhashi K, Izumi Y, Kurane R . A novel enzyme, 2'-hydroxybiphenyl-2-sulfinate desulfinase (DszB), from a dibenzothiophene-desulfurizing bacterium Rhodococcus erythropolis KA2-5-1: gene overexpression and enzyme characterization . Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1598 . 1–2 . 122–30 . July 2002 . 12147352 . 10.1016/s0167-4838(02)00365-5 .
Watkins LM, Rodriguez R, Schneider D, Broderick R, Cruz M, Chambers R, Ruckman E, Cody M, Mrachko GT . Purification and characterization of the aromatic desulfinase, 2-(2'-hydroxyphenyl)benzenesulfinate desulfinase . Archives of Biochemistry and Biophysics . 415 . 1 . 14–23 . July 2003 . 12801508 . 10.1016/S0003-9861(03)00230-3 .